Coding

Part:BBa_K1321354:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)


sfGFP fused to CBDclos driven by T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 68


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The T7 vector was fused to the protein fusion using the Xba and SpeI site.

Source

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.

References